Effects of Plant-Growth-Promoting Rhizobacteria on Soil Bacterial Community, Soil Physicochemical Properties, and Soil Enzyme Activities in the Rhizosphere of Alfalfa under Field Conditions

Background: Inoculation with plant-growth-promoting rhizobacteria (PGPR) effectively increases plant growth in agriculture. However, the role of rhizobiome remains unclear. Methods: Biolog Ecoplate and 16S rRNA gene high-throughput sequencing techniques were used to analyze changes microbial community diversity, composition, function after PGPR inoculation. Soil physicochemical enzyme activities also measured. Results: inoculation significantly promoted alfalfa. Using a Ecoplate, improved metabolic activity carbon source utilization soil microorganisms. increased diversity richness bacterial rhizosphere alfalfa relative abundance key taxa such as Arthrobacter, Sphingomonas, Bacillus, which are conducive growth. Conclusions: enriched sources beneficial for induced relevant functions contributed under field conditions.